Journal: Stem Cell Reports

Article Title: Polysaccharides from Ganoderma lucidum Promote Cognitive Function and Neural Progenitor Proliferation in Mouse Model of Alzheimer's Disease

doi: 10.1016/j.stemcr.2016.12.007

Figure Lengend Snippet: G. lucidum Polysaccharides Increase NPC Proliferation and Self-Renewal In Vitro (A and B) Monolayer adult hippocampal NPC cultures were treated with GLP of different concentrations for 24 hr in culture medium containing 1 ng/mL EGF and 1 ng/mL bFGF. EdU was added 2 hr prior to fixation. Representative images of EdU (red) and DAPI (blue) staining in the culture treated with (A, left) vehicle (Ctrl) or (A, right) 30 μg/mL GLP are shown. The percentage of EdU + cells among total cells in the culture was determined (B). n = 3 independent experiments. (C–E) Adult hippocampal NPC were cultured in neurosphere-forming conditions in the presence of absence of GLP. Six days later, the number of neurospheres (C) and cells (D) were quantified for each condition. All neurospheres from each condition were collected, dissociated, and replated in the untreated culture medium. Six days later, the number of neurospheres was determined (E). n = 4 independent experiments. (F) Adult hippocampal NPC from APP/PS1 mice were cultured and treated the same as in (B), and the percentage of EdU + cells among total cells in the culture determined. n = 3 independent experiments. (G) Human iPSC-derived NPC cultures were treated with GLP of different concentration for 6 days in neurosphere-forming conditions. Neurospheres were quantified (n = 4 independent experiments). Quantifications are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 analyzed by one-way ANOVA followed by Fisher's protected least significant difference test. Scale bars, 100 μm. See also Figure S3 .

Article Snippet: Human iPSC-derived NPC were provided by IxCell Biotechnology and the monolayer cell culture was maintained in neural stem cell culturing medium (IxCell) in Matrigel (Corning)-coated dishes.

Techniques: In Vitro, Staining, Cell Culture, Derivative Assay, Concentration Assay

Journal: Nature protocols

Article Title: Generation of spatial-patterned early developing cardiac organoids using human pluripotent stem cells

doi: 10.1038/nprot.2018.006

Figure Lengend Snippet: Troubleshooting guidelines

Article Snippet: Cell type (Step 27) The expected results shown in this protocol were obtained using a WTC hiPSC line (available at Coriell Institute NIGMS Human Genetic Cell Repository) donated from Bruce R. Conklin at the Gladstone Institute.

Techniques: Construct, Clinical Proteomics, Cell Culture, Generated, Software